Extradiol oxygenase

There is an additional problem that has important implications for the bioremediation of contaminated sites when two substrates such as a chlorinated and an alkylated aromatic compound are present. The extradiol fission pathway is generally preferred for the degradation of alkylbenzenes (Figure 9.17), although this may be incompatible with the degradation of chlorinated aromatic compounds since the 3-chlorocatechol produced inhibits the activity of the catechol-2,3-oxygenase (Klecka and Gibson 1981 Bartels et al. 1984). 

Block DW, F Lingens (1992b) Microbial metabolism of qninoline and related compounds XIV. Purification and properties of lH-3-hydroxy-4-oxoquinoline oxygenase, a new extradiol cleavage enzyme from Pseudomonas putida strain 33/1. Biol Chem Hoppe-Seyler 370 343-349. 

The extradiol-cleaving oxygenases, which catalyze the reactions in eqs. (7) and (8), are more abundant than the intradiol enzymes, but they are less stable because of their sensitivity to oxidizing compounds. Catechol 2,3-dioxygenase (2,3-CTD) is known as metapyrocatechase and was recognized in 1961 by Kojima et al. [117] and crystallized by... 

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