Experimental design detection capability

Despite its clever utility, SELDI suffers from several limitations. The SELDI MS instrumentation usually is capable of accurately detecting proteins with molecular weights less than 45,000, the detected proteins cannot be identified using this technique alone, and reproducibility in complicated experiments is suspect (86). Improvements in next-generation instruments using ProteinChip tandem MS techniques that enable direct protein identification (87), improved surface chemistries (88), and improved experimental design (89,90) should all greatly enhance SELDI s effectiveness as a powerful proteomic tool (91,92). 

Traditionally, the design of the injection channel has always been in the form of a cross. A limitation of this configuration is that it is only capable of providing discrete, fixed-volume samples. However, some applications require the delivery of different sample volumes to the detection and separation processes. The unique multi-T configuration injection system [3] has the ability to simulate the functions of the cross, double-T, and triple-T systems through appropriate manipulations of the electric field within its various channels. Figure 7 shows the experimental and numerical simulation results of the proposed multi-T form injection system. 

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