Experiment size exclusion chromatogram

This presentation demonstrates that a small minicomputer can be used to provide a full range of functions for collection and interactive reduction of data from a size-exclusion liquid chromatograph. A number of different users have collected in excess of 5000 chromatograms using this equipment. The experience gained with this system has influenced our approach to the automation of other analytical instruments. Careful attention to control paths, provision of "user friendly" access to the system functions, and careful management of the data archiving functions are crucial to the success of such efforts. 

Fig. 1. Chromatograms illustrating difference between a small zone (a) and a large zone (b) size exclusion experiment. In the saull zone experiment, the elution volume, V, is taken as the apex of the peak In a large zone experiment, the elutxon volume is the centroid volume, V, of the leading boundary. The shaded areas represent the volume loaded onto the column in each case.

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