EXPERIMENT 4 GEL PERMEATION CHROMATOGRAPHY USING DUAL DETECTORS UV AND Rl

EXPERIMENT 4 GEL PERMEATION CHROMATOGRAPHY USING DUAL DETECTORS (UV AND Rl) 

Objective Background Additional materials Safety and disposal Experiments 

Discussion Self-help questions Additional experiments 

The objective of this experiment is to calibrate a gel permeation chromatographic column and to separate a series of small molecules by GPC. Additionally, this experiment will demonstrate the usefulness of a spectrophoto-metric and RI detector in GPC preparative separations. 

Gel permeation chromatography can be considered as a mechanical sorting of sample molecules according to differences in their effective size in solution. As the sample (solute) molecules travel through the column they 

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