Excited-State Ionization of Naphthol

The spectral shifts that occur upon di ocisl Hi in the excited state can be used to calculate the change in that 

While the FSrster cyele calculation is useful for undostanding the exdted-state ionization of fluorophores, the calculated values of ApR , should be used with caution. This is because at 300 nm an error of 4 nm cnrresponds to a shift of one pK, unit Hence, the Forster cyde is useful 

Figiure183. Companson of grBoad tide (---------) and eicited-state 3.1992, 247-249 Copyright C 1992, Divistoa of CheaiBcalEdBca- 

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