Examples of PSDF and Phase Suppression

Since the initial reports on PSDF. this method has been applied to a variety of samples. These applications are illustrated by two examples, suppression of scattered light and resolution of an excited-state reaction. 

Scattered light has a zero Ufedme and is thus always Ottt-of-(diase to some extent with the enussion. Sevtf al laboratories have suggested the use of phase-sensitive detection tosi ipress Raman orRayfeighscatteredlj t Hus 

Hgure 22.7. Steady state emission spectrum of quinine soifaie (----- 

I F can be used to record the emission spectra of (MOteiiK. Hus is illustrated In Figioe 22.8 for a mixture of the neutral analogs of tryptophan and Cosine. At a detector phase angle of 103 , the tyrosine emission is 

One can examine such samples using phase-sensitive spectra. The basic idea is to consider the short-wavelength side of die emission as representing die initially excited state and the red side of die emission as rq esenting the solvenwelaxed state. Suppression of the emission on each side of the emission spectrum allows die apparent relaxed 

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