Examples of Biocatalyst Purification

In the following examples, typical purification protocols are covered and explained. 8.6.1 

Example 1 Alcohol Dehydrogenase [(R)-ADH from L. brevis (Riebel, 1997)] 

The purification table (Table 8.4) can be regarded as typical for a novel biocatalyst with unknown properties. 

Step Specific activity [Umg- l Yield pi] Total activity M Purification factor [fold] 

5 -ADP-Sepharose affinity column to pick up NADP-dependent enzymes. Source Riebel (1997). 

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Examples of Biocatalyst Purification P 239 Table 8.5 Purification table for L-amino acid oxidase from Rhodococcus opacus. 

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