Evolution and applications of HPLC

Apart from the reduction in the scale of the quantities separated, two other areas of progression should be noted [Pg.87]

Flow-rate 0.5 mL/min T = 30°C MS Detection Mobile phase, water and acetonitrile, gradient elution. [Pg.88]

The use of stationary phases adapted to particular separations sugars, PAH, amines, nucleotides... [Pg.88]

1 Current HPLC apparatus can use columns of internal diameter (written as ID in the text), of 300 p.m, and for which the optimum flow rate advised is 4p.L/min. [Pg.89]

Show by simple calculation that this flow rate will conduct the mobile phase at practically the same linear speed as in a column of the same type but possessing a standard diameter of 4.6 mm for which the advised flow rate is 1 mL/min. [Pg.89]

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