Events of ATP Formation

There are two types of ATP bound to F tightly bound but exchangeable ATP molecules that represent catalytically active intermediates (or transitory bound ATP), and practically nonexchangeable tightly bound ATP. In the course of enzyme turnover, nucleotides bound to noncatalytic centers of a subunits do not escape from the enzyme molecule, although ATPsynthase 

All the data available point to ADP as the immediate acceptor of P. There are no other phosphorylated precursors other than ADP, thus, ATP is the first compound formed from in the course of photophosphorylation in chloroplasts and chromatophores, and oxidative phosphorylation in mitochondria. The data obtained in several laboratories demonstrated that adenine nucleotides bound to can incorporate P from the medium, forming [ PJ ATP labeled immediately in the y position [147-151]. 

Mitchell proposed, the osmotic protonmotive force may be applied directly to the hydroxide or oxide ion, pulling it into the catalytic center of.  

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