Evaluation of Separation Methods

To evaluate and optimize separation methods for overlapping peaks, there is a software tool called hilchromet. With hi chromet, the analyst has a tool for determining how well the chosen separation method in his chromatography data system really is it deals with symmetric as well as a symmetric peaks. 

The aim of hi chromet is to create a comprehensive evaluation report about the usability of different separation techniques for overlapping symmetrical and tailing peaks. This leads to the documented knowledge of how accurately the separation method used really is, what mistakes you make when using individual separation methods, and which peak parameters the individual peaks of the chromatogram really have. 

The chromatogram and the determined integration results are imported and a simulation of the chromatogram is created. The simulation is adapted by means of visual shift. Deviation parameters determine the comparability with the original chromatogram. The report on the processed chromatogram provides an overview of the deviations of each separation method used. In addition, he receives a recommendation for which separation method he should apply for individual peaks overlapping, to obtain the least deviation. 

In the chromatogram of the following example, consisting of six separation peaks, peaks 3 and 4 overlap the most. Peaks 1, 5, and 6 elute baseline 

the analyst is faced with the questions as to which of the available separation method he should use and which errors the values can have. 

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These are the interesting points of the chromatogram for the use and evaluation of separation methods and deconvolution. 

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