Eukaryotes spheroplasts

The removal of 30-35 % PolyPs from yeast cells during the lysis of cell walls by the snail enzyme was observed in Saccharomyces carlbergensis (Vagabov et al., 1973) - these were alkali-soluble fractions (Table 5.3). A comparative investigation of the amounts of various PolyP fractions was carried out in E. magnusii spheroplasts (Table 5.4.) and some sub-cellular fractions (Table 5.5). All of these data confirm the idea of PolyP localization in different compartments of cells of the lower eukaryotes. 

The fuselloviruses of crenarchaeota are much less likely to be fusogenic for crenarchaeotic and prokaryotic spheroplasts, than the L3 A. laidlawii phage would be. The cell division mechanisms of crenarchaeota vide supra), especially those of the thanmarchaeon ( miraculous ) Nitrosopumilus maritimus, and eukaryotes are phylogeneticaUy related (by the shared operon cvd ESCRT FtsZ) [42, 43], yet even these ingredients cotrld not have created eukaryotic cells by their presumed... 

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