Ethylvinyl ketone

The substrate used for assay purposes was diethyl maleate. Among the most active substrates were w-nitrostyrene and 3,4-dimethoxy- J-sty ene, ethylvinyl ketone, hexene-l-ai, and cycIohex-2-ene-l-one. The enzjTne is closely associated with the other glutathione transferases in rat liver preparations. When a rat liver supernatant was dialyzed, 50% of the activities of glutathione S-alkyitransferase and iS poxidetransferase were lost but over 80% of the jS-alkenetransferase was retained. The enzyme has not been obtained free of substantial amounts of the jS-aryltransferase. 

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