Ethylsuccinic acid

Ethylester (trinitro) deriv of ethylsuccinic acid, bis-trinitro 6 E293—E294... 

The phenyl group appears to be essential, since succinic acid and ethylsuccinic acid are not attacked by the reagent. 

See., stc.-Butylmalonic Acid. 2-Methylbutane-l 2-dicarboxylic Acid. See 1-McthyJ-l-ethylsuccinic Acid. 2-Methylbutane-l 3 dicarboxylic Acid. 

Methyl-l-ethylsuccinic Acid 2-MethyU buiane-l 2-dicarboxylic acid, isoperUane-l 2-dicarboxylic acid)... 

Ethylsuccinic acid ( C H, qOi, ) ( ) a-Rethyl-o-ethylsuccinic acid (CyHizOi,) ( )... 

Radioactive la obtained after feeding methionine-methyP C to 5. lincolnensis var. lincolnensis fermentation, was degraded to MTL and ethylhygric acid (EHA). The EH A was found to be twice as radioactive as the MTL. After oxidation of the EHA with permanganate, radioactive ethylsuccinic acid was isolated, having a level of radioactivity equivalent to that of propylsuccinic acid derived from the radioactive PHA referred to above. 

Since the ratio of radioactivity of EHA MTL in la is the same as that of PHA MTL in lincomycin, it is concluded that the ——CH3 and —S—CH3 groups are derived from methionine in the same fashion. Oxidation of the EHA to still radioactive ethylsuccinic acid is taken, by analogy to the results with PHA, to mean that the C—CH3 of EHA also originates in methionine. It thus appears that lincomycin and la originate by the same pathway from intermediates differing by one carbon atom. 

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