Ethylenediaminetetraacetic acid ratio

In buffered solutions containing ethylenediaminetetraacetic acid, PC PG vesicles with molar ratios 9 1, 4 1, and 1 1 behave in the same way as pure PC vesicles, that is, the pulses induce opening of macropores with a diameter up to about 10 pm, which reseal within tens of milliseconds. In nonbuffered solution, the membranes with low fractions of charged lipids (9 1 and 4 1) retain this behavior, but for membrane composition of 1 1 PC PC, the vesicles collapse and disintegrate after electroporation [142] (see Figure 7.6). Typically, one macropore forms and expands in the first 50-100 ms at a very high speed of approximately 1 mm s" . 

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