1,2-Ethylenediamine with sucrose

Apart from the synthetieally useful methods of synthesis described above, derivatives of this ring system have been obtained in low yields by a variety of methods. Aminolysis of sucrose with ethylenediamine, " pyrolysis of actinomycin Pip-2, and photochemical degradation of di-quat have all produced pyrido[l,2-a]pyrazines. Hydrogenation of... 

Notes To purify nucleic acids and proteins, an initial subcellular fractionation may be advantageous. In this case the lysis buffer (e.g. 50 mM tris-HCl, pH7.5) generally contains dithiothreitol (DTT), ethylenediamine tetraacetate (EDTA) and/or protease inhibitors such as phenylmethylsulfonyl fluoride (PMSF) in isotonic sucrose (0.25M sucrose, d = 1.0317). To purify polysaccharides, cells may be lysed/extracted with organic solvents (e.g. n-butanol) or alkali. 

Polyols with a large number of terminal hydroxyl groups result when the reaction is initiated with compounds such as pentaerythritol, 2,2,6,6-tetrakis(hydroxymethyl) cyclohexanol, or with natural products, for example, sucrose, raffinose, or D-mannitol. Amines such as ethylenediamine, diethylenetriamine, and 2,4-diaminotoluene may also be used as the active hydrogen-containing initiator. 

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