Ethanol acidic, solution preparation, 95 percent

Ethanol formulations of salicylic acid (20 and 30%) are used for combination peeling (see salicylic acid section). Trichloroacetic acid is prepared as an aqueous solution, since ethanol solutions do not penetrate the skin. It is prepared by mixing the appropriate concentration of crystals with up to 100 cc of distilled water. Ten and fifteen percent TCA is prepared by mixing 10 or 15 g of crystals in up to 100 cc of total volume, respectively. Aqueous solutions of TCA remain stable for up to 6 months unless contaminated. Other methods have been used to formulate TCA peeling solutions however, the weight/volume methods appear to be the most reliable formulation [5]. Premixed TCA solutions are available from a variety of medical... 

Prepare a solution containing 3-5 percent water in ethanol (v/v) and adjust the pH to 4.5-5.5 with acetic acid. 

Anthranilic acid esters 50mg of glafenine are dissolved in 5mL of ethanol. 1 mL of diluted sulphuric acid is added. The mixture is cooled for 2 minutes in an ice bath. 1 mL of 1 % millimole per volume of sodium nitrite solution is added and the mixture is left for 10 minutes in the ice bath. 1 mL of freshly prepared and filtered B - naphthol solution of 10% millimole per volume in concentrated ammonia, is added. The obtained solution is compared in colour intensity with a simultaneously prepared standard solution of a mixture of 4.8mL of ethanol and 0.2 mL of methyl anthranilate solution (0.05% millimole per volume in alcohol). The percent concentration of anthranilic acid esters in glafenine should not exceed 0.2% (3). 

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