Essential information about the antibody

3 Problems with purifying antibodies from cuiture supernatant 

1 Low concentration of antibody in culture supernatant compared with serum 

If a low-secreting cell line is the best available and the antibody is important, the only option is to grow a large quantity of cells and to concentrate the culture supernatant before further processing. These objectives can both be accomplished in a hollow fibre fermenter. Alternatively, supernatant from conventional cultures can be concentrated in a variety of devices. Hollow fibre kidney dialysis cartridges are particularly convenient, being reliable, free from endotoxin, easy to use, and cheap. 

Concentration of culture supernatant using a hollow fibre device 

Peristaltic piunps of choice (e.g. Masterflex with size 17 pump head) 

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