Escherichia coli honey

A number of allergens from both honey bee and vespid venoms have been cloned and expressed by either Escherichia coli or baculovirus-infected insect cells (table 1) phospholipase Aj [20], hyaluronidase [21], acid phosphatase [13] and Api m6 [14] from honey bee venom, as well as antigen 5 [22], phospholipase A and hyaluronidase [23] from vespid venom, and dipeptidylpeptidases from both bee and Vespula venoms [15, 16]. Their reactivity with human-specific IgE antibodies to the respective allergens has been documented [11-16, 22, 23] and their specificity is superior... 

Dudler, T., Chen, W.-Q., Wang, S., Gmachi, M Dempcy, R., Annand, R., Crameri, R., Suter, M., and Gelb, M. H. (1992). High-level expression in Escherichia coli and rapid purification of enzymatically active phospholipase A2 from honey bee venom. Biochim. Biophys. Acta 1165,201-210. 

Sherlock, O., Dolan, A., Athman, R., Power, A., Gethin, G., Cowman, S., Humphreys, H., 2010. Comparison of the antimicrobial activity of Ulmo honey from Chile and manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Bmc Complementary and Alternative Medicine 10 (1), 47. 

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