Enzymes reaction with, phosgene

Carbamates, like carbosulfan (66), have a similar mode of action, namely poisoning of the enzyme acetylcholinesterase, except that this is now achieved by carbamoylation rather than phosphorylation. Thiolocarbamates like (70) are prepared from the appropriate amines by reaction with phosgene (Scheme 13). 

A group of activation reagents similar to GDI have been developed in Israel (11). They resemble phosgene and prodnce immobihzed enzymes with bonds identical to those produced by GDI. One of their advantages is that p-nitrophenyl chloroformate can be used to prepare an activated matrix that has the property of releasing the yellow p-nitrophenol anion as conphng occurs, thereby permitting one to easily monitor the reaction visnally ... 

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