Enzymes particle creation

To increase dispersion or surface area of salt-enzyme preparations in organic media, a method of preparing surfactant-assisted salt enzyme nanodispersions (SSENDs) was explored. The goal was to create a fine dispersion of the salt enzyme lyophilized particles with the use of a siufactant prior to lyophilization by creation of a fine emulsion, followed by removal of solvent and water by lyophilization. Microscopic observation of the emulsion indicated a submicron dispersion of the aqueous phase in the solvent. The results of the ertzyme assay using SSENDs catalyst is shown in Table 3. This experiment was conducted in hexane to determine if such a preparation can enhance activity of the enzyme because posdyophilization addition of the surfactant did not improve the rate of the reaction. The results show that a two- to threefold higher rate can be obtained by preparation of the salt-SC using the SSENDs preparation method. 

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