Enzymes P-galactosidase

Parameters Radiometric proximity assays (SPA, Flashplate) Fluorescence polarization (FP) Time- resolved fluorescence (HTRF) Amplified luminescence (ALPHAScreen) Enzyme (p-galactosidase) complementation Electrochemilumines cence... 

Targeted enzyme p galactosidase peptidase esterase, lipase sulfa tase reductase... 

An enzyme P-galactosidase catalyzes the hydrolysis of a substrate p-nitorophenyl-P-D-glucopyranoside to /i-nilrophenol, the concentrations of which are given at 10, 20, and 40 min in the reaction mixture, as shown in Table P3.6. Table P3.6 Time course of enzyme reaction... 

Cloned Enzyme Donor Immunoassay. This technique, abbreviated CEDIA, has arisen as a result of recombinant DNA technology, and a fundamental understanding of the enzyme p-galactosidase. This enzyme is active only in its native tetrameric structure the four identical subunits comprising the tetramer are inactive if the quaternary structure of the holoenzyme is absent. It is now known that if any of the amino acids between positions 10 and 60 from the N-terminal end of the p-galactosidase subunit are absent, an active tetramer cannot be formed. 

Kinetic spectrophotometric assays for Na are based on activation of the enzyme p-galactosidase by Na" to hydrolyze o-nitrophenyl-[3-D-galactopyranoside (ONPG). The rate of production of o-nitrophenol (the chromophore) is measured at 420 nm. 

Recombinant E.coli bacteria bearing plasmid with one of the following promoters dnak, grpE, ovfabA were nsed. These promoters were fnsed to the reporter enzyme P-galactosidase [5, 7]. Ethanol [1%] or phenol [1.6 ppm] was introduced to the... 

Roels et al. [55] and Buchet et al. [98] observed a slightly higher prevalence of elevated urinary excretion of albumin, transferrin, retinol binding protein and the tubular enzyme P-galactosidase in chloralkah workers with a urinary excretion of mercury exceeding 50 fig/ g creatinine. 

The key feature of a plasmid capable of blue/white screening is the gene for the a-subunit of the enzyme p-galactosidase. These plasmids are used with a strain of E. coli that are deficient in the a-subunit of this enzyme. Galactosidase can convert a colorless sugar derivative, called X-gal, to a... 

Synthesis of Sialyated Trisaccharides. We have used a combination of glycosidases and glycosyltranferases to prepare sialylated trisaccharides (Equations 8-9). The enzyme P-galactosidase from bovine... 

The following protocol uses the relatively uncommon ELISA detection system of the enzyme p-galactosidase plus the substrate, 4-methylumbellifeiyl (3-galacto-side, which is enzymatically hydrolysed to the highly fluorescent product 4-methylumelliferone. This stem has several favourable features ... 

Some fluorogenic substrates have become popular of late. These are methyl umbilllferyl galactoside for the enzyme P-galactosidase and methyl umbilllferyl phosphate for the enzyme alkaline phosphatase. 

Galactooligosaccharides (also called transgalactooHgosaccharides) are obtained from lactose using the galactosyltransferase activity of the enzyme P-galactosidase, which is reflected in solutions... 

Lactose is determined using two immobilized enzymes P-galactosidase and glucose oxidase [39]. The P-galactosidase catalyses the hydrolysis of lactose ... 

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