Enzymes exocellular hydrolytic

MODELLING OF PHYSIOLOGICAL CONTROL OF PRODUCTION OF EXOCELLULAR HYDROLYTIC ENZYMES... 

The fed-batch technique of cultivation is limited by fermenter volume because the volume of fermentation broth increases after each substrate addition. This disadvantage can be avoided by application a cyclic feeding strategy [9-11] which allows one to control the synchrony of individual cell development [9] by the limiting substrate concentration. The preliminary simulation results on a computer show that this type of environmental control of continuous production of exocellular hydrolytic enzymes based on our model is very promising. 

A fundamental question involves the biocidal activity of the biocide when it is chemically attached to a polymer. Will the polymer be an active biocide If the biocidal activity results from an interaction of the biocide at the cell wall or by inactivating an exocellular enzyme, then it is possible that the polymer. Itself, could be an active biocide. However, if the biocide must be incorporated into the organism to function, then it will have to be cleaved from the polymer binder prior to exhibiting activity. For that reason polymers having biocides attached by functional groups of differing hydrolytic suscepta-blllty were made. 

In summary, the main mechanistic studies of P-lactam antibiotics with penicillin-sensitive enzymes have been performed with soluble exocellular enzymes which act as hydrolytic catalysts. Unfortunately, mechanistic studies with membrane-bound PBPs which act as penicillin-sensitive enzymes are far less advanced and so the molecular mechanism for the lethal action of P-Iactam antibiotics remains speculative. 

This transformed relation was applied to the production of exocellular lipase [7], The values of R /jjX were plotted against X - X in Figure 6, The course of the function describing the influence of environment on the production of lipase is comparable with the dependences for the limiting substrate in Figures 4 and 5, Equation 6 for enzyme production simplifies the model of physiological control and can be applied in various situations for evaluation and computer simulation of hydrolytic enzyme production,... 

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