Enzyme molybdenum-containing

These enzymes may contain other redox-active sites (iron-sulfur centers, hemes, and/or flavins), either in distinct domains of a single polypeptide or bound in separate subunits. These additional cofactors perform electron transfer from the molybdenum center to an external electron acceptor/donor. 

Sulfate reducers can use a wide range of terminal electron acceptors, and sulfate can be replaced by nitrate as a respiratory substrate. Molybdenum-containing enzymes have been discovered in SRB (also see later discussion) and, in particular, D. desulfuricans, grown in the presence of nitrate, generates a complex enzymatic system containing the following molybdenum enzymes (a) aldehyde oxidoreduc-tase (AOR), which reduces adehydes to carboxylic acids (b) formate dehydrogenase (FDH), which oxidizes formate to CO2 and (c) nitrate reductase (the first isolated from a SRB), which completes the enzy-... 

Goughian, M., Ed. Molybdenum and Molybdenum Containing Enzymes Perga-mon Press, Oxford, 1980. 

Bauder R, B Tshisuaka, F Lingens (1990) Microbial metabolism of quinoline and related compounds VII. Quinoline oxidoreductase from Pseudomonas putida a molybdenum-containing enzyme. Biol Chem Hoppe-Seyler 371 1137-1144. 

De Beyer A, E Lingens (1993) Microbial metabolism of quinoline and related compounds XVI. Quinaldine oxidoreductase from Arthrobacter spec. Rii 61a a molybdenum-containing enzyme catalysing the hydroxylation at C-4 of the heterocycle. Biol Chem Eloppe-Seyler 374 101-120. 

Koenig K, JR Andreesen (1990b) Xanthine dehydrogenase and 2-furoyl-coenzyme A dehydrogenase from Pseudomonas putida Fnl two molybdenum-containing enzymes of novel structural composition. JBacteriol 111 5999-6009. 

Table 1. Some molybdenum-containing enzymes. For some of the enzymes the numerical data are rather approximate only. Turnover numbers refer to temperatures between 23 and 30°. Since some of the molecular weights are particularly uncertain, contents of flavin etc. are expressed per molybdenum atom rather than per mole of protein. Most of the enzymes have a much wider range of substrate specificities than has been indicated...

Turnover numbers of molybdenum-containing enzymes generally tend to be low. A brief discussion of each of the enzymes in Table 1 is given below. 

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