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Enzyme-linked immunosorbent cleavage

Accurate measurement of proinsulin has been difhcult for several reasons the blood concentrations are low antibody production is difficult most antisera cross-react with insulin and C-peptide, which are present in much higher concentrations the assays measure intermediate cleavage forms of proinsulin and reference preparations of pure proinsulin are not readily available. However, a more sensitive nonequiUb-rium RIA method for measuring proinsiilin was developed by adsorbing the initial antiserum with biosynthetic human C-peptide coupled to agarose to eliminate cross-reactivity with C-peptide.An enzyme-linked immunosorbent assay (ELISA) has been described that employs an antibody to C-peptide as the coating antibody and antiinsulin antibody for detection. The detection limit is 0.25 pmol/L. ... [Pg.851]

Detection of Internucleosomal Cleavage by Enzyme-Linked Immunosorbent Assay (ELISA). Induction of apop-... [Pg.166]

See other pages where Enzyme-linked immunosorbent cleavage is mentioned: [Pg.6]    [Pg.466]    [Pg.66]    [Pg.47]    [Pg.224]    [Pg.142]    [Pg.174]    [Pg.327]    [Pg.2181]   
See also in sourсe #XX -- [ Pg.129 ]



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Cleavage enzyme

Immunosorbent

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