Enzyme-immunohistochemistry procedures in electron microscopy

The essential difference between light and electron microscopy is the degree of resolution. To obtain this higher resolution in electron microscopy, it is essential to have a better preservation of the ultrastructure. This requirement creates at the same time an obstacle for the demonstration of intracellular antigens by EIH, since the penetration of antibodies or of antibody-enzyme conjugates through membranes decreases considerably upon improved preservation. 

Only those methods will be discussed which are pertinent to ultra-structural EIH and supplement the usual techniques employed in electron microscopy. Many of the concepts discussed above also apply in electron microscopy. 

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