Enzyme Assays Pepsin Activity

Lipase (Microbial) Activity for Medium- and Long-Chain Fatty Acids, (S3)105 Lysozyme Activity, (S3)106 Maltogenic Amylase Activity, 804 Milk-Clotting Activity, 805 Pancreatin Activity, 805 Pepsin Activity, 807 Phospholipase A2 Activity, 808 Phytase Activity, 808 Plant Proteolytic Activity, 810 Proteolytic Activity, Bacterial (PC), 811 Proteolytic Activity, Fungal (HUT), 812 Proteolytic Activity, Fungal (SAP), 813 Pullulanase Activity, 814 Trypsin Activity, 814 Enzyme Assays, 786 Enzyme-Hydrolyzed (Source) Protein,... 

Additional substrate was added at 30 min intervals for multiple incubation experiments. At the end of the experiments, the enzyme solution was dialyzed against 3 1 of 0.01 M sodium acetate buffer, pH 4.0, for 72 hr with 5 changes. Hydroxamic acid was quantitatively analyzed by the method of Bergmann and Segal, (21). Pepsin activity was determined by the hemoglobin assay method described by Chow and Kassell (20). 

A protocol to improve pepsin immobilization was proposed by Busby et al. [17], Rather than directly using pepsin, they immobilized pepsinogen to POROS AL-20 support under optimal coupling conditions of pH 6.7 where pepsinogen is stable (in contrast with pepsin). Subsequently, they converted the coupled pepsinogen into active pepsin. Protein assays demonstrated that more enzyme was bound to the POROS AL-20 resin coupled with pepsinogen at pH 6.7 than pepsin at pH 5. 

Active immobilized enzyme determination of heat changes in the proximity of immobilized enzymes with an enzyme thermistor, and the use of an enzyme thermistor in assays for metabolites Flowing-stream analyser for measuring penicillin in fermentation broths Active immobilized enzyme studies of the kinetic properties and stability of immobilized pepsin... 

---