Enzyme alkali-soluble cellulose from

Figure 9. Effect of enzyme components from T. koningii cellulose on the formation of alkali-soluble cellulose from cotton fiber. A reaction mixture consisting of 200 mg of cotton, 2 mL of 0.2 M acetate buffer, pH 4.5, enzyme, water, and NaNs solution to give a total volume of 10 mL, was incubated at 50°C for various times. Percentage hydrolysis was calculated from the loss in weight determined on weighed crucibles. The fraction soluble in alkali (10% NaOH) was determined in a similar fashion after incubating for 3 hr at 25°C. Ct ( - 0) Cx + /3-glucosidase ( - ) ...

$Figure 9. Effect of enzyme components from T. koningii cellulose on the formation of alkali-soluble cellulose from cotton fiber. A reaction mixture consisting of 200 mg of cotton, 2 mL of 0.2 M acetate buffer, pH 4.5, enzyme, water, and NaNs solution to give a total volume of 10 mL, was incubated at 50°C for various times. Percentage hydrolysis was calculated from the loss in weight determined on weighed crucibles. The fraction soluble in alkali (10% NaOH) was determined in a similar fashion after incubating for 3 hr at 25°C. Ct ( - 0) Cx + /3-glucosidase ( - ) ...$

The situation is complicated by the fact that the efficiency of cellulose synthesis in the cell-free system is low, and the same enzyme preparation catalyzes the incorporation of D-glucosyl groups from UDP-Glc into alkali-soluble (1 - 2)-/3-D-glucans.370 A similar process was reported to occur with a membrane preparation of Rhizobium meliloti,371... [Pg.326]

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