Transglycosylation, enzymatic

Scheme 32. Enzymatic transglycosylation of a preformed bisglucobipyridine core self-assembled as a hexavalent cluster around a ruthenium cation.272...

Scheme 4 6 -0-Acyl-lactose (acetyl, propionyl, butyryl) derivatives prepared via the selective enzymatic acylation of lactose by the protease subtilisin were used as acceptors for enzymatic transglycosylations catalyzed by a-D-galactosidase from Talaromyces flavus, forming iso-globotriose a-Gal(l -> 3)-p-Gal-(l -> 4).92 (/) Protease N, pyridine, 37 °C (ii) a-galactosidase from Talaromyces flavus.

Glycosyl Azides Novel Substrates for Enzymatic Transglycosylations... 

Glycosyl Azides Novel Substrates for Enzymatic Transglycosylations 233 propan-1-o1 H20 NH3 aq., 7 2 1... 

A series of other glycosides of ergot alkaloids was prepared by enzymatic transglycosylations using activated p-nitrophenylglycosides as donors [47]. 

Fig. 10 Chanoclavine mannosylation using enzymatic transglycosylation and reversed glycosylation...

Thiamin (vitamin Bi, 22) (Fig. 14) - an important cofactor of decarboxylases, transketolases, carboxy-lyases, and some other enzymes - was successfully glycosylated by enzymatic transglycosylation using p-galactosidase [59] and p-A-acetyl-hexosaminidase [60] from A. oryzae. 

Trincone, A., Nicolaus, B., Lama, L. and Gambacorta, A. (1991) Stereochemical studies of enzymatic transglycosylation using Sulfolobus solfataricus. J Chem Soc Perkin Trans, 1, 2841-2844... 

Figure 21 Polymerizable disaccharide 48 synthesized via a one-pot enzymatic transglycosylation catalyzed by a-D-galactosidase from green coffee beans.

The synthesis of 4-0-p-D-glucopyranosyl-moranoline by enzymatic transglycosylation is referred to in Chapter 3. 

As shown in Figure 1, the enzymatic transglycosylation and hydrolysis are a competing process. The efficiency to form a new oligosaccharide or glycoconjugate by the enzymatic reaction depends on the intrinsic transglycosylation activity of the endoglycosidase and on the control of the... 

Mori T, Li M, Kobayashi A, Okahata Y (2002) Reversible control of enzymatic transglycosylations in supercritical fluoroform using a lipid-coated P-D-galactosidase. J Am Chem Soc 124 1188-1189... 

Kanno K, Maeda H, Izumo S, Ikuno M, Takeshita K, Tashiro A, Fujii M (2002) Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactor. Lab Chip 2 15-18... 

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