Enzymatic peak-shift technique

A method of great value in biochemistry for the verification of peak identities is the enzymatic peak shift technique.5 The approach utilizes the specificity of enzyme reactions with a nucleotide or class of nucleotides. The technique is especially useful in the characterization of nucleotides of cell extracts, because not only is the identity of the reactant verified, but so is the identity of the product formed. With the enzyme peak shift method, one aliquot of the sample is analyzed while a second aliquot is incubated with an excess of an enzyme that catalyzes a specific reaction involving the compound of interest. After the enzyme is deactivated, the second aliquot is chromatographed. 

The enzymatic peak-shift technique has been widely used for the identification of compounds or classes of compounds in biological matrices... 

In the enzymatic peak-shift technique, the following criteria must be met ... 

Enzymatic or chemical peak-shift technique (use of a specific chemical or enzymic reaction to remove selected components of the mixture)... 

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