Enzymatic activity dual output molecular probe

DUAL OUTPUT MOLECULAR PROBE FOR ENZYMATIC ACTIVITY... 

Dual Output Molecular Probe for Enzymatic Activity 151... 

A molecular probe with dual output signals offers two detection modes allowing use of the same probe in different environments. We have demonstrated how an AB2 self-immolative dendron with double quinone methide release mechanism can be applied to create a molecular probe with UV-Vis and fluorescence modes for the detection of a specific catalytic activity.15 The molecular probe is illustrated in Fig. 5.36. The central unit of the probe (the molecular adaptor) is linked to an enzymatic substrate that acts as a trigger and to two different reporter molecules. Cleavage of the enzymatic substrate triggers the release of the two reporters and a consequent activation of their signals. 

FIGURE 5.36 A graphical illustration of a molecular probe for detection of enzymatic activity with dual output. (See the color version of this hgure in Color Plates section.)... 

Danieli, E. Shabat, D. Molecular probe for enzymatic activity with dual output. Bioorg. Med. Chem. 2007, 15, 7318-7324. 

FIGURE 5.36 A graphical illustration of a molecular probe for detection of enzymatic activity with dual output. 

---

See also in sourсe #XX -- [ ]

---