Energy Transfer in Membranes

In these equations exp[-o5(0] describes that portion of the donor decay doe to RET, a is the surface densi of the acceptor, and rc is the distance of closest approach between the donor and accqitors. The energy-transfer ciency can becalcolaled by an equation analogous to Eqs. [13.13] and [13.14], except that the intensities or lifetimes are calculated from integrals of the donor intensity decay  

One may readEfy visualize how energy qu icfaing data could be used to detennine ndiedier the lributi is of donor and accepter are random. Using the calculmed vahie ofRo one compares the measured extent of donor quench- 

There is considerable infcxmation in these time-resolved decays, and methods to recover this information are described in Chapter 15. In the more general case, the distance between donor and acceptor can vaiy both as a result of a range of distances and by difiusion. Both factors affect the rates of eneigy transfer and must be considered in any such analysis. 

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