Endoproteinase LysC

Endoproteinase LysC cleaved AP-A between Lys37 and Ala38 to yield a derivative with cardiotonic activity an order of magnitude lower than that of the parent molecule (Monks SA and Norton RS, unpublished results). This reduction in activity could be a consequence of local conformational perturbations. Treatment of AP-B with carboxypeptidase removed Lys49, resulting in only a two-fold reduction in cardiotonic activity (Monks SA and Norton RS, unpublished results). 

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