Endopeptidases engineering

J.-B. Charbonnier and A. M nez, Engineering cyclophilin into a proline -specific endopeptidase, Science 1998, 391, 301-304. 

It is interesting to study whether and how an engine degrades polycationic macromolecules in the presence of a polyanion. An endopeptidase, namely trypsin, that was previously shown to be capable of degrading preferentially PLL while keeping intact the PLCA and PLCAI nylon-type polyamides was selected (7). All the PEC fractions were destabilized in a 0.1 M NaH2P04 and 1.9 M NaCl solution at pH = 7.9 and at 25°C, before introduction into a 1,000 Da cut-... 

Wehofsky, N., Wissmarm, J., AUsch, M., Bordusa, F. (2000). Engineering of substrate mimetics as novel-type substrates for glutamic acid-specific endopeptidases design, synthesis, and application. Biochim. Biophys. Acta, 1479,114-122. 

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