Enantioselective Liquid Chromatographic Analysis of Amino Acids

ENANTIOSELECTIVE LIQUID CHROMATOGRAPHIC ANALYSIS OF AMINO ACIDS 

Most amino acids have a chiral center at the alpha position, resulting in L- and D-enantiomers. High-performance liquid chromatography is the most widely used technique for the separation of amino acid enantiomers. For that purpose, various chiral deiivatizing reagents and chiral stationary phases are frequently used. The following topics are t)rpical examples of the LC separation and determination of amino acid enantiomers. 

l Fluoro 2,4 Diiiitroplienyl 5 L-Alanine Amide (Marfey s Reagent) 

FIGURE 6.5 Reaction of chiral derivatization reagents with amino acid enantiomers. 

Cyclodextrins (CDs) are useful selectors for the separation of enantiomers, and various chiral stationary phases containing a CD moiety have been reported. Armstrong et al. reported the direct enantiomer separation of several amino adds using an a-CD bonded stationary phase 

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