Emission Spectra Taken through Filters

Hguie 2.21. Rejection of acatteied from the 8 tryptophan station in Rgnre 2.20 u ng a bandpass filter (fep) or a pdarlwbut no bant Mss filt (bottom). The enussion polaciaw was oriented verileally (H) or horizontally (X). From Ref. 8. 

Polarization or anisotropy measurements are frequently performed using filteis rather than monochtomatois. Scattered light is 100% polarized (r = 1.0). Hence, a small percentage of scatter can result in serious ertois. For example, assume 10% of the observed intensity is due to Raman scatter. Furthermore, assume that the actual anisotropy of a sample, in the absence of scatter, is 0.10. The observed anisotropy is given by 

FIgHie 2.22. Embsioo spectn of trypKH um with t trace iin Nirity of tyrosine, otedned with excitadon at 275 and 295 nm. Rom Ref. 8. 

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