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Embryonic development ecdysteroids

To verify in vitro results, we have also developed an in vivo assay for L. dispar PTTH (52, Thyagaraja et al, in preparation). This assay is comparable to larval assays develop for Af. sexta (56 and B. mori (58) and uses last instar female larvae. Neck ligation prior to day 7, the day when a small pre-peak in hemolymph ecdysteroid titer appears, blocks further development. Injection of PTTH-containing extracts posterior to the ligation, including post-embryonic egg extract, reinitiates development and subsequent pupation. Attempts to develop an in vivo assay comparable to the pupal-adult B. mori assay (5B were unsuccessful, since brain removal as close as 15 min post-pupal ecdysis failed to block L. dispar female adult development (Thyagaraja et a/., unpublished results). [Pg.29]

See other pages where Embryonic development ecdysteroids is mentioned: [Pg.5]    [Pg.170]    [Pg.7]    [Pg.22]    [Pg.222]    [Pg.197]    [Pg.305]    [Pg.30]    [Pg.221]    [Pg.300]   
See also in sourсe #XX -- [ Pg.3 ]

See also in sourсe #XX -- [ Pg.3 ]



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