Eluent cupric sulfate

Figure 7.9. Separation of metal ions on a PRP-X200 column. 1.50 X 4.1 mm. Eluent 3.5 mM cupric sulfate, indirect UV detection at 220 nm (Courtesy Hamilton Co).

There are certainly many other possibilities for direct spectrophotometric detection. A separation of several divalent metal ions is shown in Fig. 7.9 with 3.5 mM cupric sulfate as the eluent and indirect detection at 220 nm. 

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