Eluant succinic acid

Fig. 3 -125. Comparison between the retention behavior of inorganic anions and several carboxylic acids. -Separator columns 2 lonPac AS4 eluant 2.8 mmol/L NaHCOs + 2.2 mmol/L Na2COj flow rate 1.6 mL/min detection suppressed conductivity injection 50 pL solute concentrations A) 1.5 mg/L fluoride (1), 2 mg/L chloride (2), 5 mg/L each of orthophosphate (3) and bromide (4), 10 mg/L nitrate (5), and 12.5 mg/L sulfate (6), B) 5 mg/L formic acid (7), 40 mg/L benzoic acid (8), 20 mg/L succinic acid (9), 10 mg/L malonic acid (10), 20 mg/L each of maleic acid (11), tartaric acid (12), and oxalic acid (13).

Figure 9 A synthetic mixture of water-soluble carboxylic acids separated by anion-exchange chromatography. Column 0.3 cm x 300 cm Diaoion CA 08, 16-20 p (Mitsubishi Kasei Kogyo). Eluant 200 mM HC1. Detection reaction with Fe3-benzohy-droxamic acid-dicyclohexy carbodiimide-hydroxylamine perchlorate-triethyl amine with absorbance at 536 nm. Analytes (1) aspartate, (2) gluconate, (3) glucuronate, (4) pyroglutamate, (5) lactate, (6) acetate, (7) tartrate, (8) malate, (9) citrate, (10) succinate, (11) isocitrate, (12) w-butyrate, (13) a-ketoglutarate. (Reprinted with permission from Kasai, Y., Tanimura, T., and Tamura, Z., Anal. Chem., 49, 655, 1977. 1977 Analytical Chemistry).

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