Eluant citrate buffer

Fig. 5-27. Separation of a standard of physiologically relevant amino acids on a totally sulfonated cation exchanger BTC 2710. - Column temperature four-step temperature program from 48°C to 70°C eluant 5 lithium citrate buffer flow rate 0.3 mL/min detection see Fig. 5-25 solute concentrations 10 nmol each of Pser (1), Tau (2), PETA (3), Urea (4), Asp (5), OH-Pro (6), Thr (7),...

Eluants.—Citric acid buffered with ammonium citrate is the first eluant to be used in the separation of rare earths by ion exchange process. It is also the most extensively [85—89] investigated eluant. At low pH the individual rare earths move down the column at different rates. A plot of volume of eluted portion vs. concentration shows typical bell-shaped curves (Fig. 2) with widely spaced maxima characteristic of elution chromatography, although the system makes use of a chelating agent. 

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