Ellipticity from sedimentation data

Most probable settling velocity from sedimentation data Particle-size determination from sedimentation equation Sedimentation in an ultracentrifuge Solvation and ellipticity from sedimentation data Diffusion and Gaussian distribution Temperature-dependence of diffusion coefficients... 

EXAMPLE 2.4 Solvation and Ellipticity of Human Hemoglobin from Sedimentation Data. The diffusion coefficient of the human hemoglobin molecule at 20°C is 6.9 10 11 m2 s "1. Use this value to determine f for this molecule. Evaluate f0 for hemoglobin using the particle mass calculated in Equation (35). Indicate the possible states of solvation and ellipticity that are compatible with the experimental flfQ ratio. 

Contours like this are qualitatively the same sort of thing we obtain from sedimentation-diffusion experiments as shown in Figure 2.9. Therefore let us consider the relationship between the two types of data. In general, exactly the same factors affect both the intrinsic viscosity and the friction factor ratio, but the functional dependencies are somewhat different. Figure 4.13b shows how a contour of f/f0 selected from a sedimentation-diffusion study and an intrinsic viscosity contour selected on the basis of viscosity experiments might overlap. In this case the solvation-ellipticity combination is characterized unambiguously a/b = 2.5 and (ml b/m2) = 1.0. Figure 4.13b shows the complementarity of viscosity and sedimentation-diffusion data. 

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