Electrophysiology whole-cell patch-clamp

The electrophysiological technique used to measure changes in membrane capacitance is the patch clamp [5,6] in the whole-cell recording mode, where the plasma membrane patch in the pipet is ruptured. In another configuration of the patch clamp, the plasma membrane patch is maintained intact. In this case, small currents due to the opening of individual channels can be measured in the membrane patch. The whole-cell patch clamp... 

The main procedure to relate the electrophysiological properties of cortical neurons to their morphological features is represented by the whole-cell patch clamp recording on slices maintained in vitro. The detailed description of this procedure is beyond the purpose of the present chapter. However, a brief overview of the main steps involved is given below. 

Live-Cell Microscopy for GFP Fluorescence Detection and Taipeting for Whole-Cell Patch Clamp Electrophysiology... 

The patch clamp technique suddenly became the workhorse of modern electrophysiology, and the whole-cell configuration became one of the most crucial and popular techniques for the biophysical and pharmacological study of ion channels. 

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