Electrophoretic theory resolution

Simple one-dimensional electrophoretic separation techniques can only partially resolve the complex mixtures of proteins and peptides found in biological fluids. Resolution may be improved by combining an electrophoretic separation with a second separation method at right angles. The second method will preferably be based on a different analytical principle. In theory, the resolution of such combinations is the product of the resolution of the component methods. 

In the early 1980s, Jorgenson and Lukacs discussed the theory and basis for electrophoretic separations in capillaries. The following brief discussion develops, albeit at a basic level, the theoretical concepts describing the movement of charged molecules in a buffer-filled capillary under an applied electrical field and the shape of the zone during the electrophoretic process. The separation process is discussed in terms of resolution (how adequately two components are separated) and efficiency (how long the separation takes). Finally, we discuss factors that affect the resolution. For more detailed evaluation of these processes, the reader is referred to several excellent discussions on this subject. ... 

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