Electrophoretic studies sodium dodecyl sulfate

Lin et al. [95] used capillary electrophoresis with dual cyclodextrin systems for the enantiomer separation of miconazole. A cyclodextrin-modified micellar capillary electrophoretic method was developed using mixture of /i-cyclodextrins and mono-3-0-phenylcarbamoyl-/j-cyclodextrin as chiral additives for the chiral separation of miconazole with the dual cyclodextrins systems. The enantiomers were resolved using a running buffer of 50 mmol/L borate pH 9.5 containing 15 mmol/L jS-cyclodextrin and 15 mmol/L mono-3-<9-phcnylcarbamoyl-/j-cyclodextrin containing 50 mmol/L sodium dodecyl sulfate and 1 mol/L urea. A study of the respective influence of the /i-cyclodcxtrin and the mono-3-(9-phenylcarbamoyl-/i-cyclodextrin concentration was performed to determine the optical conditions with respect to the resolution. Good repeatability of the method was obtained. 

For some studies, washed lipid globules can be used as such. More commonly, it is necessary to dissociate membranes from globules by chemical or physical methods. Chemical methods normally involve direct extraction of constituents from the globules. Sodium dodecyl sulfate solutions have been used to recover membrane proteins from washed lipid globules for subsequent electrophoretic characterization (Kobylka and Carraway 1972 Mather and Keenan 1975). Other workers have used solutions of detergents such as deoxycholate (Hayashi and Smith 1965) and Triton X-100 (Patton 1982). With these deter-... 

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