Electrophoretic separation in agar gel

It follows that electroosmotic flow decreases with a lowered dielectric constant and with an increased viscosity of the liquid phase. This connection is more pronounced when working in agar gel tihan in filter paper because, in agar, electroosmosis is much stronger. We have added to 1% agar gel 25 % by volume of 1,2-propanediol, whereupon the viscosity of the agar sol increases from 1.8 to 4.4 in tiie 

All the factors which cmitribute to a good separation in unit time must be well considered because the interpretation of the final precipitate lines is much facilitated if the original distance of separation was sufficiently long. 

We have outlined in the foregoing section the most important factors which contribute to electrophoretic fractionation in agar gel. It will be easier now to look at the basic relation which exists between the charge of the particle and the migration velocity (Bl), The electrophoretic migration of colloidal particles follows Eq. 4  

If the observed substance has migrated as an anion, Uo, must be added, and it must be subtracted in the case of a cation. Details of such controls are specified under technique in Section 6. 

Where the ampholyte, as depicted in the center, is widrout net charge against the medium, the isoelectric point is reached at which the am- 

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