Electrophoretic Characterization of Isozymes Zymograms

Isozymes are enzymes that catalyze the same biochemical reactions but differ based on molecular weight. For example, the electrophoretic mobility of lactate dehydrogenases (LDH), enzymes responsible for the 

Compared with other molecular methods, isozyme profiling can be relatively inexpensive to perform (Loureiro and Querol, 1999). Isozyme detection should be performed at various intervals during growth because the proteins being detected may or may not be expressed by the microorganism at similar times. 

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