Electrophoresis malate dehydrogenase

Fig. 18.2. Enzyme patterns of some strawberry species Fragaria sp.) and Fragaria ananas) obtained by PAGE disc gel electrophoresis. Large pore concentrating gel pH 6.7, small pore separating gel, pH 8.9. 1 Peroxidase incubation with o-toluidine/H202 at pH 7. 2 Esterase incubation with a-naphthylacetate at pH 7, the released a-naphthol is diazotized and then coupled with p-chloroaniline. 3 Malate-dehydrogenase incubation with malate, nitro-blue-tetrazolium chloride and NAD at pH 7.5. (according to Drawert et al 1974)...

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