Electronic reference to access in vivo

The referencing can also be done by employing an artificial reference resonance. The electronic reference to access in vivo concentrations also known as the (ERETIC) method provides a reference signal, synthesized by an electronic device, which can be used for the determination of absolute concentrations. The ERETIC approach has been applied to ID NMR quantification with high accuracy the standard deviation has been reported to remain imder Michel and... 

When seeking to determine the concentration of a solute by NMR spectroscopy, it is always necessary to measure it against that of a known caUbrant. T)rpically, this would demand the addition of a suitable reference compound to the anal)rte solution against which the integrated intensity of a solute resonance may be compared following the protocols described above for quantitative measurements. However, the use of an internal chemical reference may impose constraints that may not be readily met, making this approach undesirable. The reference material needs to be soluble in the anal)tie solution, it must be inert to the solute, its resonance(s) must not overlap that of the solute and its relaxation properties should be similar to that of the solute. In addition to these practical considerations, one simply may not wish to add a reference contaminant to a precious sample. An alternative approach to this use of an internal chemical reference is to introduce a synthetic tf reference signal as in the ERETIC method (Electronic REference To access In Vivo Concentrations) [7, 8]. 

ERETIC Electronic reference to access in-vivo conventrations 4.1.3... 

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