Electron microscopy cryosections

Reid N, Beesley JE. Sectioning and Cryosectioning for Electron Microscopy, Elsevier, New York, 1991. 

Erk I, Nicolas G, Caroff A, Leparet J. Electron microscopy of frozen biological objects a study using cryosectioning and cryosubstitution. J Microsc 1998 189 236-248. 

Slot, J. W. and Geuze, H. J. (1984) Gold markers for single and double immuno-labeling of ultrathin cryosections, in Immunolabelling for Electron Microscopy (Polak, J. M. and Vamdell, I. M., eds.), Elsevier, New York, pp. 129-142. 

A tissue section cut from a frozen specimen in this situation, ice is the supporting matrix. See Yamada, E. and Watanabe, H., High voltage electron microscopy of critical-point dried cryosection, J. Electron Microsc. 26 (SuppL), 339-342, 1977 Maddox, P.H., Tay, S.K., and Jenkins, D., A new fixed cryosection technique for the simultaneous immuuocytochem-ical demoustratiou of T6 and SlOO antigens, Histochem. J. 19, 35-38,... 

Figure 5.40. Transmission electron microscopy thin section of phosphotungstic acid stained cryosections of an as extruded (A) and deep drawn (B) coextruded film constructed of toughened nylon/barrier resin/tough-ened nylon. (From Wood [186] used with permission of the American Chemical Society Rubber Division.)...

Figure 5.70. A phase contrast optical micrograph (A) of an impact modified nylon shows the fine dispersion of modifier in the matrix. Transmission electron microscopy micrographs of a cryosection, stained with ruthenium tetroxide (B and C), show more detail and finely dispersed subinclusions (arrows) within the elastomeric phase. Scanning transmission electron microscopy (D) of an unstained cryosection shows less dense regions in a darker background due to mass loss of the rubber phase during exposure to the electron beam, resulting in contrast enhancement.

The preparation ofultrathin cryosections (cryoultramicrotomy) for transmission electron microscopy (EM) offers a number of advantages such as highest sensitivity, the possibility of using osmium tetroxide as a counterstain after immunolabeling, and the study of, e.g., the intracellular pathways of neuropeptide secretion [37, 38]. However its high cost and technical difficulties have greatly limited its diffusion. In addition, only very small areas of... 

Fig. 5.47 The dispersed phase morphology in an impact modified nylon is shown by phase contrast microscopy (A) and by TEM of a cryosection stained with ruthenium tetroxide (B). A STEM micrograph (C) shows regions that are less dense in a dark background. The dispersed phase structure suggests that there is more mass loss in the rubber phase than in the nylon matrix during exposure to the electron beam, permitting imaging of the particles without staining.

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