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Electron mediator-free detection

A simple and inexpensive cholesterol biosensor was fabricated by immobilizing cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) onto a poly (thionine) modified glassy carbon electrode (GCE/PTH) (Fig. 41) [122]. Hydroqui-none (HQ) was used as a mediator to promote the electron transfer between the enzyme and the electrode. It results in excellent electrocatalytic activity of immobilized HRP for H2O2 reduction, which was produced from cholesterol by the enzymatic reaction with ChOx. The linear range for cholesterol spanned from 25 to 125 iM, with a detection limit and a sensitivity of 6.3 pM and 0.18 pA/cm /p M, respectively. The highly reproducible and sensitive GCE/PTH/ChOx/HRP sensor exhibited an interference-free signal for cholesterol detection with excellent recoveries for real sample analysis. [Pg.415]

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See also in sourсe #XX -- [ Pg.156 ]

See also in sourсe #XX -- [ Pg.156 ]



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