Electroenzymatic oxidation with dehydrogenases

Fig. 4. Electroenzymatic oxidation of / -D-glucose with direct electrochemical NAD+ regeneration (GDH = glucose dehydrogenase)...

Scheme 11. Idealized sketch showing the electroen matic oxidation of L-lactate at gold modified electrode surfaces, (a) Lactate dehydrogenase bound to CB-terminated alkylthiol SAMs prepared by covalent attachment of CB to 3-mercaptopropionic acid SAM derivatized with 1,4-diaminobutane. The electroenzymatic oxidation of lactate is observed only in the presence of soluble coenzyme (NAD" ") and a redox mediator (phenazine methosulfate) [215]. (b) Lactate deh3tdrogenase bound to NAD-terminated alkylthiol SAMs prepared by covalent attachment of Af -(2-aminoethyl)-NAD to a cystamine SAM derivatized with pjrrroloquinoline quinone. The reconstituted enzyme is electrically wired to the electrode surface via two NAD" -binding pockets involved in the affinity-binding surface reaction [242].

Figure 21. Electroenzymatic oxidation of w e o-diols to give enantiomerically pure lactones with horse liver alcohol dehydrogenase (HLADH) and PDMe" as redox catalysts at pH 7.8 and 150mV vs. Ag/AgCl reference [125].

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